Composite

Part:BBa_K3286011:Design

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-16)


sgRNA expression cassette (double target)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References